Journal: Genes & Cancer

Article Title: CD133-positive cancer stem cells from Colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile

doi:

Figure Lengend Snippet: A-B. Cytometry analyses of Nestin, CK20, CD133, Oct3/4, CD44 and CD44v9 on 10% FBS Colo205 growing cells (control) and serum-free growing cultures (week 1 to week 5).

Article Snippet: The purity of sorted cells was evaluated by flow cytometry using FACSCalibur (BD Biosciences) after labeling with anti-human CD133/2 or CD44 antibody (Miltenyi Biotec).

Techniques: Cytometry, Control

Journal: Genes & Cancer

Article Title: CD133-positive cancer stem cells from Colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile

doi:

Figure Lengend Snippet: A. Tumorsphere evaluation of CD133+ and CD133- sorted cells. B. Invasiveness of different cell fractions measured using a collagen-based invasion kit (relative DO measured at 560 nm). C. Survival assay after chemotherapy treatment with cisplatin and 5-FU. D. Metabolite quantification after CE-TOF-MS experiments. Only metabolites of interest for Colo205 cells cultured in 10% FBS (control; green bar), CD133+ sorted cells (red bar) and CD44+ sorted cells (yellow bar) are reported here.

Article Snippet: The purity of sorted cells was evaluated by flow cytometry using FACSCalibur (BD Biosciences) after labeling with anti-human CD133/2 or CD44 antibody (Miltenyi Biotec).

Techniques: Clonogenic Cell Survival Assay, Cell Culture, Control

Journal: PLoS ONE

Article Title: CD166/ALCAM Expression Is Characteristic of Tumorigenicity and Invasive and Migratory Activities of Pancreatic Cancer Cells

doi: 10.1371/journal.pone.0107247

Figure Lengend Snippet: (A) qRT-PCR analysis of the mRNA levels of CD166 in SUIT-2 cells after RNA interference was performed at the indicated days post-transfection. Control (siControl) or CD166 silenced cells (siCD166) were analyzed by (B) colony formation assays at the indicated days post-transfection. (C) qRT-PCR analysis of EMT markers E-cadherin, N-cadherin, Zeb-1, and MMP2 in CD166+ and CD166- Panc-1 and SW1990 cells. (D) The relationships between expression of CD166 and CSC markers CD24, CD44, and CD133 in Panc-1 cells were analyzed by flow cytometry. Data represent the mean ± SD; *, p <0.05; NS, not significant.

Article Snippet: Cells from subconfluent monolayer cultures were suspended in phosphate-buffered saline (PBS) and incubated with monoclonal anti-human ALCAM-phycoerythrin (PE) (R&D Systems, Minneapolis, MN), anti-human CD24-fluorescein isothiocyanate (FITC) (eBioscience Inc, San Diego, CA), anti-human CD44-FITC (MBL, Nagoya, Japan), and monoclonal anti-human CD133-FITC (Ancell Corp, Bayport, MN) antibodies.

Techniques: Quantitative RT-PCR, Transfection, Expressing, Flow Cytometry

Journal: International journal of oncology

Article Title: Isoform switch of CD44 induces different chemotactic and tumorigenic ability in gallbladder cancer.

doi: 10.3892/ijo.2017.4063

Figure Lengend Snippet: Figure 1. (A) Histogram of cell surface CD44 expression in the human gallbladder cancer cell line NOZ. (B) Double-staining analysis of CD44std and CD44v9. CD44std+-CD44v9- (CD44s, orange) cells and CD44std--CD44v9+ (CD44v, blue) cells were sorted. (C) Morphology of sorted NOZ-CD44v and CD44s cells cultured under normal conditions. (D) Reverse transcription-PCR analysis of mRNAs encoding CD44s, CD44v9, and CD44 (as well as pan-CD44) expressed by the sorted NOZ-CD44v and CD44s cells (*p<0.001).

Article Snippet: Cell surface proteins were detected using an allophycocyanin (APC)-conjugated antihuman CD44 monoclonal antibody (clone BD105, catalog number 130-095-177; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), a fluorescein isothiocyanate (FITC)conjugated anti-human CD44 standard monoclonal antibody (CD44std; clone SFF-2, catalog number BMS113FI; Affymetrix Inc., Santa Clara, CA, USA), and an anti-human CD44v9 rat monoclonal primary antibody (clone RV3, catalog number LkG-M001; Cosmo Bio Co., Ltd., Tokyo, Japan).

Techniques: Expressing, Double Staining, Cell Culture, Reverse Transcription

Journal: International journal of stem cells

Article Title: Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion.

doi: 10.15283/ijsc24079

Figure Lengend Snippet: Fig. 1. Characterization of human umbilical cord mesenchymal stem cells (hUCMSCs). (A) Morphology of fourth-generation hUCMSCs under light microscope, red arrows represent the typical parts, the magnification is 10×. (B, C) Detection of multidirectional differentiation potential (B into chondrogenic differentiation, C into adipogenic differentiation) characteristic of hUCMSCs, red arrows represent the typical parts, the magnifications are 10×. (D-I) Flow cytometry detection of positive markers (CD29, CD105, CD90, CD44) and negative markers (CD45, CD34) characteristic of human umbilical cord MSCs.

Article Snippet: The cells were incubated with 5 μL of antihuman CD105, CD34, CD45, CD29, CD44, and CD90 antibodies (Elabscience) in the dark for 30 minutes.

Techniques: Light Microscopy, Flow Cytometry